Possible ID: Absidia
Classification:
Phylum: Zygomycota
Class: Mucoromycotina
Order: Mucorales
Family: Cunninghamellaceae
Isolation and culturing methods:
Isolated from soil taken from outside of the gym. It had recently rained and the soil was hardening but still moist. Sample was obtained through serial dilution C onto a plate with malt extract agar. Plates were stored at room temperature for a week then hyphae was transferred to a new plate in order to obtain a pure culture.
Culture appearance and growth:
White hymenium growing upwards on the surface. Loosely spread across the surface. Fast growth rate (plate covered in 1 week with sores being produced). Hyphae about 2.3-2.6 microns wide, no septate, no clamp connections.
Spore production:
Zygospores are 2.2-2.5 x 1.3-1.5 microns and develop on the tops of the hyminium. Spore are not impeded in agar. Appear black to naked eye, little coloration when under the microscope. Fairly round, slight oval shape.
Collector: Regina Group: Seneca and Regina
Top label: #39