Sample ID #: 32 Possible ID: Penicillium Classification: Phylum Ascomycota Class Eurotiomycetes Order Eurotiales Family Trichocomaceae Species? Location: This sample was collected off of a moldy strawberry allowed to mold for a week in a petri dish. Isolation and culturing methods: A square section (around 0.5 x 0.5 cm) of the primary mold sample was extracted from its substrate and cultured on a malt extract agar plate for two weeks. A small section of the hyphae of this culture was then extracted and placed onto a MEA plate to grow a pure culture. Description of macroscopic and microscopic characteristics: Hyphae […]

Possible ID: Sordariomycota sp. Classification: Ascomycota Class: Sordariomycetes     Isolation and culturing method: Isolated from specimen container that was left outside for 2 hours in The Grove Park, Lewisburg PA. The sample was then grown in culture for 2 weeks and then removed from the plate and placed into a container for growth on its own. These plates were in a room at room temperature and allowed to grow off of Agar plates for sustenance. After trying this process 2 times the pure culture was finally arrived at a pure culture.   Culture appearance and growth: The culture appears […]

ID: Schizophyllum   Phylum: Basidiomycota Class: Agaricomycetes Order: Agaricales Family: Schizophyllaceae   Collected from Turtle Creek Park on April 2nd. It had recently snowed and the ground was covered. Specimen was found on wood and taken back to lab for identification.   The Fruiting body appeared to have gills on its underside. However, upon further examination under a dissecting microscope, gills turned out to be more like “folds”. This is an identifying feature in the Schizophyllum genus. Fruiting body was small and fan shaped, covered in small white hairs. Genus tends to be sabrobic on decaying wood, and occasionally parasitic […]

Possible ID: Nigrospora sp.   Classification: Phylum: Ascomycota Class: Sordariomycetes Order: Trichosphaeriales Family: Incertae sedis Genus: Nigrospora   Isolation and culturing methods: Isolated from a plate placed under an air vent in the men’s bathroom on the third floor of the Rooke Biology building for around 10 minutes on February 19th of 2018. After being stored at room temperature for four weeks along with a multitude of other fungi, hyphae of a black fungal sample were taken from this plate and placed onto its own malt extract agar plate. After 3 weeks, a block of hyphae at the edge of […]

Stephanie Harper Bio373 Mycology Pure Culture Identification Blog   Possible ID: Rhizopus stolonifer   Classification: Phylum: Zygomycota Class: Mucoromycotina Order: Mucorales Family: Mucoraceae   Isolation and Culturing Methods: This culture was isolated from an agar plate growing various molds from a rotting wood sample. Specimen was allowed to grow in malt extract agar + chloramphenicol (MEA+C) for one week at room temperature, then hyphae that were not threatened by contamination were moved to a new sterile MEA+C plate and allowed to grow for another week.   Culture Appearance on Growth: In the agar plate, hyphae and sporangium are visible to […]

Possible ID: Penicillium sp.   Classification: Phylum: Ascomycota Class: Eurotiomycetes Order: Eurotiales Family: Trichocomaceae   Isolation and Culturing Methods: This specimen was isolated from soil samples in the Rooke Greenhouse, from a planter pot housing a Chenopodium oahuense plant. The soil was given a Nitrogen/Phosphorus blend four weeks prior to collection, and the greenhouse is kept at 26*C, >20% humidity and nightly UV light treatments. Specimen was grown in malt extract agar + chloramphenicol (MEA+C) for one week at room temperature, then hyphae that were not threatened by contamination were moved to a new sterile MEA+C plate and allowed to […]

Stephanie Harper Bio373 Mycology Pure Culture Identification Blog   Possible ID: Trichoderma sp.   Classification: Phylum: Ascomycota Subphylum: Pezizomycotina Class: Sordariomycetes Order: Hypocreales Family: Hypocreaceae   Isolation and Culturing Methods: This culture was isolated from mold growing on a piece of cooked chicken left out at room temperature for several days. Specimen was allowed to grow in malt extract agar + chloramphenicol (MEA+C) for one week at room temperature, then hyphae that were not threatened by contamination were moved to a new sterile MEA+C plate and allowed to grow for another week.   Culture Appearance on Growth: Hyphae radiate from […]

Sample ID: 57 Possible ID: Absidia sp.   Classification: Phylum Zygomycota Class Zygomycetes Order Mucorales Family Cunninghamellaceae   Isolation and culturing method: Isolated from moss on a wood log. The sample placed on malt extract agar + chloramphenicol. Plate was left alone for a couple of weeks and then a small volume of hyphae was excised from the agar and transferred to achieve a pure culture.   Culture appearance and growth: Fungus grew slowly. After a couple of weeks of growth, culture was white, fuzzy, and cotton-like. Edge of culture was light green. Hyphae grew above agar, pointing upward. Hyphae […]

Sample ID: 59 Possible ID: Daldinia sp.   Classification: Phylum Ascomycota Class Ascomycetes Order Xylariales Family Xylariaceae   Isolation and culturing method: Isolated from grass tissue. The grass sample was cut in half and placed flat on malt extract agar + chloramphenicol. Plate was left alone for a couple of weeks and then a small volume of hyphae was excised from the agar and transferred to achieve a pure culture.   Culture appearance and growth: Fungus grew quickly. After one week of growth, culture was white and fuzzy. After a few more weeks of growth, culture turned grey-brown with clumps […]

Sample 33 Possible ID: species in Pezizomycotina   Classification: Phylum Ascomycota Subphylum Pezizomycotina   Isolation and Culturing Methods: This sample was collected from a soil sample outside of the biology building on Bucknell’s campus on March 3rd, 2018. The sample of soil was directly plated onto a MEA plate and cultured for two weeks. The sample was extracted from the soil sample plate and cultured on a new plate and grew for one week. This process was repeated an additional time to ensure its isolation.     Description of macroscopic and microscopic characteristics: Hyphae regularly septate, without clamp connections, 1-2 […]