Sample ID: 56 Possible ID: Aspergillus sp.   Classification: Phylum Ascomycota Class Eurotiomycetes Order Eurotiales Family Trichocomaceae   Isolation and culturing method: Isolated from an air sample taken on top of a heater in the Rooke Biology building basement for 3 hours. The sample was serially diluted with water and plated on malt extract agar + chloramphenicol. Plate was left alone for a week and then a small volume of hyphae was excised from the agar and transferred to achieve a pure culture.   Culture appearance and growth: Grey/brown colored fungus grew quickly, but experienced no growth or overgrowth after […]

Possible ID: Aspergillus flavus   Phylum: Ascomycota Class: Eurotiomycetes Order: Eurotiales Family: Trichocomaceae   Isolation and culturing methods: Air sample taken in between Rooke Chemistry and Van Lit. for seven minutes. Air sample was taken on a plate with malt extract agar plus antibiotics. Air sample was stored at room temperature for one week in order to let collected culture grown. After one week, samples were taken and the isolates were transferred from the main plate to an new one with the same agar conditions. Isolate was stored at room temperature for one week in order to obtain pure culture. […]

Possible ID: Trichoderma viride   Phylum: Ascomycota Class: Sordariomycetes Order:Hypocreales Family: Hypocreaceae   Isolation and culturing methods: Air sample taken in the Grove for seven minutes. Air sample was taken on a plate with malt extract agar plus antibiotics. Air sample was stored at room temperature for one week in order to let collected culture grown. After one week, samples were taken and the isolates were transferred from the main plate to an new one with the same agar conditions. Isolate was stored at room temperature for one week in order to obtain pure culture.   Culture appearance and growth: […]

Possible ID: Absidia sp., potentially Absidia corymbifera   Classification: Phylum: Mucuromycotina Class: Zygomycetes Order: Mucorales Family: Mucoraceae Genus: Absidia   Isolation and culturing methods: Isolated from a plate placed under an air vent in the men’s bathroom on the third floor of the Rooke Biology building for around 10 minutes on February 19th of 2018. After being stored at room temperature for a week along with a multitude of other fungi, hyphae of the sample were taken from this plate and placed onto its own malt extract agar plate. The following week, hyphae were removed from the new plate and transferred […]

Possible ID: Aspergillus sp.   Classification: Phylum: Ascomycota Class: Eurotiomycetes Order: Eurotiales Family: Trichocomaceae Genus: Aspergillus   Isolation and culturing methods: Isolated from soil that was collected near senior apartment #4 on February 25th of 2018. A small amount of the soil sample was serially diluted with water and placed on several malt extract agar plates. After being stored at room temperature for a week, hyphae from serial dilution C were transferred onto two new MEA plates in order to ensure that a pure culture would be obtained.   Culture appearance and growth: Fungal species was a somewhat blueish-green fungus […]

Possible ID: Ampelomyces quisqualis Classification: Powdery mildew Phylum: Ascomycota Class: Dothideomycetes Order: Pleosporales Family: Phaeosphaeriaceae   Isolation and culturing methods: Isolated from soil located in the Bucknell University Greenhouse, on the 4th floor of the Biology building. Sample was placed on malt extract agar + chloramphenicol and allowed to grow for a week. Pure sample was obtained by sectioning a small sample of the growth onto MEA plate.   Culture appearance and growth: Surface texture appears white and fuzzy 1wk into growth. Small black pores (>1mm in diameter) appear after 3wks of growth. Hyphae measure 4-5um wide, with septa present, […]

Possible ID: Hypomyces completus   Classification: Dikarya Phylum: Ascomycota Class: Sordariomycetes Order: Hypocreales Family: Hypocreaceae   Isolation and culturing methods: Isolated from Turtle Creek park fruiting body growing on bark. Fruiting body sample was placed on malt extract agar + chloramphenicol and allowed to grow for a week. Pure sample was obtained by sectioning a small sample of orange growth onto MEA plate.   Culture appearance and growth: Surface texture appears white and fuzzy above an orange foundation, with circular form. Early growth (1wk) shows small orange liquid droplet secretions. Hyphae measure 3.5-5um wide, and regularly septate, with no clamp […]

Possible ID: Uknown   Isolation and culturing methods: This fruiting body was collected on April 2nd in Turtle Creek Park in Lewisburg, PA. It was collected from a branch that had fallen. It was placed in a paper bag and brought back to the lab to further analyze and identify.   Culture appearance and growth It has a plane pileus shape and it has entire pileus margin. It has a glabrous pileus texture. The lamellae attachment is andante. It is a shelf fungus which does not have a stipe The hymenium is pored. Additionally, it is white and thick; it […]

Possible ID: Cladosporium   Classification: Phylum Ascomycota Class Dothideomycete Order Canodiales Family Davidiellacaeae   Isolation and culturing methods: Isolated from an agar plate that was placed near a vent in the Rooke Science at Bucknell University (Lewisburg, PA). This plate was used when we were collecting spores to differentiate from ecosystems inside and outside the lab.  Plates were incubated at 20 degrees Celsius for a week or more. Then, a small volume of hyphae was excised for the agar and transferred to achieve a pure culture.   Culture appearance and growth Before the was pure cultured, it formed one large […]

Possible ID:  Trichothecium   Classification: Phylum Acscomycota Class Sordariomycete Order Hypocreales Family Incertae sedis   Isolation and culturing methods: Isolated from an agar plate that was used for a prior lab and was labled “TriB.” However, because it was not pure, Trichothecium grew on it. A small volume of hyphae of this was excised for the agar and transferred to achieve a pure culture.   Culture appearance and growth The culture looked pink/rosy with white hyphae growing out of it. The hyphae are around 4-5 micrometers wide.   Spore production The spores are enclosed in conidiospores around 12 microns long […]