Possible ID. Botrytis sp. Classification: Phylum Ascomycota Class Leotiomycete Order Helotiales Family Sclerotiniaceae   Isolation and culturing method : A moldy strawberry was removed from the fridge and a small amount of the material growing on the fruit was transferred to a M+C agar plate. The plate was left at room temperature for one week. A small section of the hyphae was then removed and plated on another M+C agar plate to produce a pure culture. Strawberry was grown in California. Culture appearance and growth: Culture is powdery and white. Grow relatively fast, completely covering an agar plate in one […]

Possible ID Cladosporium sp. Classification: Phylum Ascomycete Class Dothideomycete Order Capnodiales Family Davidiellaceae   Isolation and culturing method : From soil found on uphill entrance of the LC. Small amount of soil centrifuged with water, plated, and grown at room temperature. Specific culture removed from plate by taking a clump of hyphae, replated four times on M+C agar plate to obtain pure culture.   Culture appearance and growth: Small, typically ½ inch circular formations, growing multiple on a plate. Individuals are septate, and possess no clamp connections. Hyphal, grows above medium not in or under. Groups are dark green and […]

Possible ID: Panellus sp. Classification: Phylum: Basidiomycota Class: Agaricomycetes Order: Agaricales Family: Mycenaceae Genus: Panellus Location: These mushrooms were collected in Pennsylvania woods on April 2nd off of a hardwood tree species. Pluerotoid mushrooms are saprobic and grow in clusters. Description: The fruit bodies are small, grow on wood (pleurotoid), have light brown gills that radiate outward from the stipe attachment, and form kidney shaped caps that are are brown along the outside and beige towards the center of the cap. The stipe is connected to the side of the cap and multiple mushrooms grow in clusters on top of […]

Sample #: 31 Possible ID: Talaromyces sp. Classification: Phylum: Ascomycota Class: Eurotiomycetes Order: Eurotiales Family:Trichocomaceae Genus: Talaromyces   Location and habitat: This sample was collected during an air spore drop collection outside of the O’leary geology building. The plates were situated under an overhang next to a mulched flower bed. The spore drop plate was left outside to collect spores for three hours. Spores were collected on 02/26/18. Isolation and culturing methods: This sample was isolated from the air spore drop plate it initially grew on and transferred onto a a MEA plate on 03/03/18. After two weeks, a small […]

Possible ID: Absidia   Classification: Phylum: Zygomycota Class: Mucoromycotina Order: Mucorales Family: Cunninghamellaceae   Isolation and culturing methods: Isolated from soil taken from outside of the gym. It had recently rained and the soil was hardening but still moist. Sample was obtained through serial dilution C onto a plate with malt extract agar. Plates were stored at room temperature for a week then hyphae was transferred to a new plate in order to obtain a pure culture.   Culture appearance and growth: White hymenium growing upwards on the surface. Loosely spread across the surface. Fast growth rate (plate covered in […]

Possible ID:  Trichoderma   Classification: Phylum: Ascomycota Class: Sordariomycetes Order: Hypocreales Family: Trichocomaceae   Isolation and culturing methods: Isolated from soil taken from outside of the gym. It had recently rained and the soil was hardening but still moist. Sample was obtained from serial dilution B onto a plate with malt extract agar. Plates were stored at room temperature for a week then extracts were transferred to a new plate in order to obtain a pure culture. Culture appearance and growth: Fungi seemed to be embedded in the agar. Center was a dark green with white rings. Hymenium was growing […]

Possible ID: Podospora   Classification: Phylum Ascomycota Class Sordariomycete Order Sordariales Family Lasiosphaericae   Isolation and culturing methods: Isolated from a soil sample that was taken from the soil right outside of the ELC at Bucknell University (Lewisburg, PA). The soil sample was serially diluted by water and then plated on an agar plate. The plate was untouched for a week and then a small amount of hyphae was taken and put on a smaller agar plate to obtain a pure culture. This extraction of hyphae was done twice to reassure that it was a pure culture and was free […]

Possible ID Sarocladium sp. Classification: Phylum Ascomycota Class Pezizomycotina Order Sordariomycetes Family Sarocladium    Isolation and culturing method From soil outside of LC. Small amount of soil centrifuged with water, plated, and grown at room temperature. Specific culture removed from plate by taking a clump of hyphae, re-plated four times on M+C agar plate to obtain pure culture.  Culture appearance and growth Surface texture looks bumpy. Central portion grows higher while external portions grow outward. Hyphae grows above agar, not in or below. Hyphae slender, about 5um wide. Septate with no clamp connections. Spore production Spore size about 10 uM […]

Classification: Phylum: Basidiomycota Class : Agaricomycete Order : Auriculariales Family: Auricularin Isolation and culturing method Found in central Pennsylvania on a mountain crest. Specifically found on a decaying log, in a gregarious cluster. Group of jelly ears are saprobic. Cluster was about waist height. Specimens are widely distributed on all continents and found during all seasons   Culture appearance and growth: Brown, gelatinous depressed pileus, fibrillose texture, crenulate margins, no lamellae. Stipe absent, attached centrally or laterally. Veined, smooth hymenium, silky or velvety to the touch. No odor, edible.

Possible ID: Aspergillus   Classification: Phylum Ascomycota Class Eurotiomycetes Order  Eurotials Family Trichomaceae   Isolation and culturing methods: Isolated from a soil sample that was taken from the soil right outside of the ELC at Bucknell University (Lewisburg, PA). The soil sample was serially diluted by water and then plated on an agar plate. The plate was untouched for a week and then a small amount of hyphae was taken and put on a smaller agar plate to obtain a pure culture. This extraction of hyphae was done twice to reassure that it was a pure culture and was free […]