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Possible ID Sarocladium sp. Classification: Phylum Ascomycota Class Pezizomycotina Order Sordariomycetes Family Sarocladium    Isolation and culturing method From soil outside of LC. Small amount of soil centrifuged with water, plated, and grown at room temperature. Specific culture removed from plate by taking a clump of hyphae, re-plated four times on M+C agar plate to obtain pure culture.  Culture appearance and growth Surface texture looks bumpy. Central portion grows higher while external portions grow outward. Hyphae grows above agar, not in or below. Hyphae slender, about 5um wide. Septate with no clamp connections. Spore production Spore size about 10 uM […]

Classification: Phylum: Basidiomycota Class : Agaricomycete Order : Auriculariales Family: Auricularin Isolation and culturing method Found in central Pennsylvania on a mountain crest. Specifically found on a decaying log, in a gregarious cluster. Group of jelly ears are saprobic. Cluster was about waist height. Specimens are widely distributed on all continents and found during all seasons   Culture appearance and growth: Brown, gelatinous depressed pileus, fibrillose texture, crenulate margins, no lamellae. Stipe absent, attached centrally or laterally. Veined, smooth hymenium, silky or velvety to the touch. No odor, edible.

Possible ID: Aspergillus   Classification: Phylum Ascomycota Class Eurotiomycetes Order  Eurotials Family Trichomaceae   Isolation and culturing methods: Isolated from a soil sample that was taken from the soil right outside of the ELC at Bucknell University (Lewisburg, PA). The soil sample was serially diluted by water and then plated on an agar plate. The plate was untouched for a week and then a small amount of hyphae was taken and put on a smaller agar plate to obtain a pure culture. This extraction of hyphae was done twice to reassure that it was a pure culture and was free […]

  Possible ID Rhizopadaceae sp. Classification: Phylum zygomycete Class mucoromycotina Order mucorales Family Rhizopodaceae   Isolation and culturing method Isolated from plate placed under air duct on the third floor of Rooke. Was on a plate with a variety of different fungi. Separated via removing small piece of hyphae and placed on a plate of just agar. Plate was left alone for one week and then a small amount was transferred onto another plate of just agar to form a pure culture.   Culture appearance and growth Plate covered in white hyphae. Growing  off surface of agar. Grows above agar, […]

Possible ID: Trichothecium Classification: Dikarya Phylum: Ascomycota Class: Sordariomycetes Order: Hypocreales   Isolation and culturing methods: Isolated from decompositions of maple leaves after 6 weeks. Sample was placed on malt extract agar + chloramphenicol and allowed to grow for a week. Pure sample was obtained by sectioning a small sample of the growth onto MEA plate.   Culture appearance and growth: Surface texture appears white and fuzzy above a dark green foundation. Early growth (1wk) shows a distinct white inner ring pattern, surrounded by a lighter green ring. Hyphae measure 4-5um wide, and regularly septate, with no clamp connections present. […]

Possible ID: Podosphaera plantaginis Classification: Podosphaera (?) Phylum: Ascomycota Class: Leotiomycetes Order: Erysiphales Family   Isolation and culturing methods: Isolated from tree bark sample 3 in mycology laboratory. Sample was placed on malt extract agar + chloramphenicol and allowed to grow for a week. Pure sample was obtained by sectioning a small sample of the growth onto MEA plate.   Culture appearance and growth: Surface texture appears white and fuzzy on top, and orange on the bottom 1wk into growth. Specimen becomes bright orange and white 3wks into growth, and excretes dark orange droplets. Hyphae measure 2.5-3um wide, regularly septate […]

Possible ID: Auricularia auricula Auricularia auricula, otherwise known as the “Jelly Ear,” is a somewhat gelatinous mushroom that is usually ear-shaped or shaped somewhat like a little cup.1,2 Consequently, because of the elastic, jelly-like texture of its flesh, this mushroom is considered to be a jelly mushroom.1 However, even though this mushroom does not taste like jelly, it is, in fact, still edible.2,3 Phylogenetically, Auricularia auricula is a basidiomycete contained in the order Auriculariales and the family Auriculariaceae.1,2 Canonically, Agaricomycetes, which are the “true mushroom-forming” fungi, once excluded jelly fungi, but after the advent of more advanced molecular techniques, it was […]

Possible ID: Rhodotorula glutinis   Classification: Phylum: Basidiomycota Class: Pucciniomycota Order: Sporidiales Family: Sporidiobolaceae   Isolation and culturing methods: Isolated from a plate that was left outside for 2 hours in The Grove Park, Lewisburg PA. Sample was put into its own plate and attempted to be put into its own pure culture multiple times over. After the 3rd time the sample had grown in a pure culture. The plates were left to grow for 1 week intervals, after each week the sample had grown greatly.   Culture appearance and growth: The surface of the culture is glossy and of […]

Sample ID: 58 Possible ID: Mucor sp.   Classification: Phylum Zygomycota Class Mucormycotina Order Mucorales Family Mucoraceae   Isolation and culturing method: Isolated from a soil sample collected from the South Campus Quad, Bucknell University, Lewisburg, PA. Sample was serially diluted in water and plated on malt extract agar + chloramphenicol. Plate was left alone for a week and then a small volume of hyphae was excised from the agar and transferred to achieve a pure culture.   Culture appearance and growth: White fuzzy appearance with fast hyphal growth. Hyphae growing above agar, with cotton candy-like appearance. Hyphae 5 um […]

Sample ID: 55 Possible ID: Trichoderma sp.   Classification: Phylum Ascomycota Class Sordariomycetes Order Hypocreales Family Hypcreaceae   Isolation and culturing method: Isolated from a soil sample collected from the South Campus Quad, Bucknell University, Lewisburg, PA. Sample was serially diluted in water and plated on malt extract agar + chloramphenicol. Plate was left alone for a week and then a small volume of hyphae was excised from the agar and transferred to achieve a pure culture.   Culture appearance and growth: Dark and scaly underneath and to the side with a central ring of lighter, fuzzy hyphae. Patchy growth […]